What are the limitations of light microscopy in resolving subcellular structures, and how can staining improve observations?

• A. Light microscopy has limited resolving power (~200 nm); staining and thin sectioning enhance contrast and reveal structures like nuclei and chloroplasts.
• B. Light microscopy can resolve structures down to 0.1 nm without staining.
• C. Light microscopy can only observe living specimens and nothing stained.
• D. Light microscopy can resolve all subcellular structures equally clearly.

Answer: (D)